Antibacterial hair removal composition

ABSTRACT

An antibacterial, non-aqueous liquid hair removing composition. The composition includes a solubilizing oil effective for solubilizing the ingredients, e.g., mineral oil, and an effective antibacterial amount of an antibacterial agent, e.g., triclosan and/or benzethonium chloride. The composition further includes botanical oils and rosins, e.g., soybean oil, gum rosin, rosin esters and titanium dioxide. The composition may also include fragrances and additional bacteriocides, e.g., phenoxyethanol. The hair removal composition is applied to a person&#39;s skin, and after a sufficient amount time, removed from the person&#39;s skin with the hair entrapped therein.

RELATED APPLICATIONS

This is a continuation application of U.S. application Ser. No.13/712,937 filed on Dec. 12, 2012 entitled Antibacterial Hair RemovalComposition, which at the date of filing this subject application willissue as U.S. Pat. No. 8,828,371 on Sep. 9, 2014, the entire disclosureof which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a non-aqueous, i.e., anhydrous hairremoval composition for the skin and its use. More specifically the hairremoval composition includes an antibacterial agent and removes hairgently and under lower temperatures than are required for wax typeformulations. The hair removal composition contains mineral oil, soybeanoil, gum rosin and rosin esters for removal of the hair and anantibacterial agent. After application to the skin, the hair removalcomposition is removed with a latex free, non-woven strip material andany remaining composition is removed with a safflower/sunflower erasinglotion.

2. Description of the Related Art

Varieties of methods have been developed for removing unwanted hair fromthe body. One typical hair removal method is “waxing”, a process bywhich a wax is used to pull unwanted hair out by the root bulb. Anapplicator is inserted into a bath of hot wax in a wax heater, withdrawnfrom the bath with the molten wax adhered thereto, and the wax isapplied to the skin. The wax cools and hardens on the hair that is to beremoved and then peeled off the body, removing the unwanted hair. Waxingis generally painful and uncomfortable for the person undergoing suchtreatment. In an attempt to minimize the pain, some technicians place acloth on top of the cooling wax. As the wax cools, it adheres to thecloth and the technician then pulls the cloth to remove the wax and thehair that has been captured by the wax. This is still painful anduncomfortable.

Other systems exist to remove body hair. Depilatory cream shaves beendeveloped that when applied to the skin degrade the hair to break andweaken the hairs. The depilatory and the degraded hair aresimultaneously removed from the skin without any mechanical assistance,e.g., by showering. This technique is gentler to the skin but is aninefficient and incomplete system for hair removal. An alternativemethod is to remove the preparation and hair by means of a spatula, toscrap the cream from the skin, along with broken hairs, and at the sametime breaking off weakened hairs, and removing those. This tends to bemore effective, but the scraping action can lead to skin coarsening androughness.

There have also been developed wax free, non-aqueous (anhydrous) liquiddepilatories that may be used. These “natural” depilatories consist ofbotanical oils and rosins. The products do not dry or stick to the skinand gently remove the hair under lower temperatures than are requiredfor wax and wax-like products that utilize constant heating above 160°F. (71° C.). Such products are sold under the NUFREE® brand (EquibalLabs, Unionville, N.Y.) and are used in conjunction with FINIPIL® brandlotion products from Equibal Labs. See also U.S. Pat. No. 7,078,050 toFusco.

The applicators used for such treatments are usually wooden applicatorsand are usually thin stick shaped applicators used for eyebrows or flat,spatula type applicators used for the skin surface, e.g., legs, arms,etc. The applicators are typically made of wood and are disposable toprevent contamination. The various State cosmetology boards demand thatapplicators that cannot be efficiently cleaned and sanitized before thenext use be discarded. Thus, most applicators are discarded after usebecause it is difficult to clean accumulated depilatory and bacteriafrom such applicators.

If reusable applicators are used, e.g., stainless steel applicators,they are usually cleaned by using heated, harsh chemicals that must beused in a well-ventilated facility. Additionally, such harsh chemicalcleaners and conditions are dangerous to customers and spa technicians.Thus, the disposable wooden applicator that is only used once, hasbecome the norm in the industry.

Applicant herein has filed a US Patent Application, U.S. Ser. No.13/607,698 filed on Sep. 8, 2012, now pending, entitled Compositions ForCleaning Applicator's for Hair Removal Compositions, the entiredisclosure of which is incorporated herein by reference. Thisapplication is directed to a non-aqueous liquid cleaning composition forapplicators, e.g., preferably stainless steel applicators, used forapplying non-aqueous hair removal compositions to the skin. Thecomposition includes solubilizing oil, e.g., mineral oil, that iseffective for solubilizing the non-aqueous hair removal composition, andincludes an antibacterial agent, e.g., triclosan.

The following is a list of US Published applications and US patentsrelated to this art:

U.S. Pat. No. 3,194,736 to Braun et al. discloses a solid depilatorycomposition that includes a sterol, a non-ionic polyalkenoxywater-in-oil emulsifier, and a solid, inert, organic, filler materialthat forms a solid base having dispersed therein a solid basic materialselected from the hydroxides, carbonates, silicates, and tribasicphosphates of the alkali and alkaline earth metals and guanidine anddepilating agents, e.g., substituted thiols. The solid, inert, organicfiller material may be a natural or synthetic wax, or solid hydrocarbon,starch, gum or resin.

U.S. Pat. No. 5,980,536 to Jamali describes a rigid, microwavablecontainer and applicator kit for body wax.

U.S. Pat. No. 6,204,230 to Taylor et al. describes aqueous antibacterialcompositions that contain a polyhydric solvent, a hydrotrope, asurfactant, an optional antibacterial agent, e.g., triclosan, and water.The compositions provide a substantial reduction, e.g., greater than99%, in Gram positive and Gram negative bacterial populations within oneminute.

U.S. Pat. No. 6,241,978 to Schlaeger describes a cosmetic compositionthat includes hydrophobic lipophilic materials as the principle vehiclecarrying the active ingredients in a continuous solid phase. Thecomposition is formed into a hairstick that is a hair fixative and mayinclude, inter alia, mineral oil, triclosan and fragrance.

U.S. Pat. No. 6,312,678 to Elliott et al. discloses an aqueous rinse-offliquid personal cleansing composition that includes about 1% to about60% by weight of a water-soluble surfactant, and 0.5% or greater of awater-soluble oil selected. Among the numerous ingredients listed, thecompositions can have mineral oil, 2-phenoxyethanol as a preservativeand triclosan as an antibacterial agent.

U.S. Pat. No. 6,599,513 to Deckers et al. describes emulsionformulations for topical application which include oil bodies and otheringredients, e.g., triclosan as an antimicrobial, and Neolone as apreservative agent.

U.S. Pat. No. 7,078,050 to Fusco discloses a white cream bacteriostatand fungicide capable of serving as a vehicle for additionalmedicaments. Also disclosed is a method for blending the ingredients,some of which are not readily compatible with each other.

U.S. Pat. No. 7,507,936 to Mast et al. describes a wax applicator stickscraper for scraping wax, and especially excess wax from a stick used inapplying wax to the skin for wax treatment and/or depilation.

U.S. Pat. No. 7,759,327 and US 2010/0249227 to Modak et al. disclosemethods and compositions which employ low concentrations of combinationsof zinc salts and antimicrobial agents in coatings for medical articlesthat contact the skin, including depilatories, waxing and razors. Thecompositions include water-soluble zinc salts, an emollient form basedon petrolatum or mineral oil, phenoxyethanol, fragrances, and triclosanand/or phenoxyethanol as antimicrobial or preservative agents.

U.S. Pat. No. 8,038,723 to Ellis et al. discloses an epilatorycomposition which is rosin-based and applied at body temperature. Theepilatory composition is provided as flat strips, comprises a gel-likematrix material, for example a rosin-based or sugar-based material and,mixed with the matrix material, a particulate material, for examplecolloidal particles of fumed silica, and a polyethylene in the form of ahomopolymer.

U.S. Pat. No. 8,124,577 to Bernhardt et al. discloses formulations ofpersonal care compositions and personal care concentrate compositions,e.g., liquid hand soaps, bath and shower washes, shampoos, facialcleaners, that contain sulfo-estolides. Such compositions includemineral oil, Neolone, triclosan and fragrances.

U.S. Pat. No. 8,157,814 to Kelsey et al. discloses an S-section plasticspatula design for depilatory application.

US 2002/0176876 to Harris et al. discloses a waterless exfoliant whichincludes triclosan for antibacterial activity. The treatment includes afirst phase composition having antibacterial, anti-inflammatory,humectant, antioxidant and exfoliant ingredients, and a second phasehaving anti-inflammatory, circulatory enhancement and prolongedmoisturizing ingredients.

US 2005/0152861 to Bruening et al. discloses a water-in-oil emulsionantiperspirant compositions containing: (a) an antiperspirant component;(b) a polyol-poly-12-hydroxystearate; (c) an oil component; and (d)water. The antiperspirant may also contain phenoxyethanol and triclosan.

US2005/0019292, US2005/0079189, US 2005/0085828 to Acher et al.discloses an epilatory composition provided as flat strips. Thecomposition comprises a gel-like matrix material, e.g., a rosin-based orsugar-based material, mixed with a particulate material, e.g., colloidalparticles of fumed silica.

US 2005/0048090 to Rau disclose an anhydrous self-warming compositionthat can be included in a depilatory cream. The composition contains atleast one inorganic or organic salt, that has a positive heat ofsolution upon contact with water and produces a warming effect on theskin. Anhydrous carriers are used, e.g., mineral oil, cotton seed oil,castor oil.

US 2006/0135627 to Frantz et al. disclose an aqueous personal carecomposition comprising titanium dioxide, triclosan, benzethoniumchloride, and depilating agents.

US 2007/0031360 to Gupta discloses a depilatory composition applied inliquid form on the surface of the skin, and which is removable bypeeling it away from the surface of the skin after setting to a pliablefilm. The composition includes (i) a pliable film forming agent, (ii) ahair binding agent, and (iii) a hydroxylic matrix forming agent.

US 2008/0004635 to McMillan et al. discloses a spatula for aiding in theremoval of a hardened wax from a person's body.

US 2009/0226384 to Mukhopadhyay et al. discloses an antimicrobialcomposition containing triclosan and at least one functionalizedhydrocarbon and/or mixtures containing such functionalized hydrocarbons.

US 2009/0285871 to Cunningham et al. discloses a germicidal wipe havinga germicidal solution of at least one peracid, at least one peroxide,and a surfactant. The solution may also have Neolone and Symocide PT.

US 2010/0158986 to Decker et al. discloses personal care compositionsand personal care products that impart perceivable aesthetic benefits ofincreased softness, quietness and drapability to the skin or hair of auser. The compositions may include a mineral oil, fragrances, andSymocide PT.

US 2010/0172847 to Modak et al. discloses preservative or antimicrobialcompositions with broad spectrum antimicrobial activity comprising lowconcentrations of essential oil and a botanical extract in synergisticcombination with a fruit acid and alkanediol, and optionally a solvent.The compositions are used in personal care products such as creams orsoap products. An antibacterial composition is disclosed that includesbotanical extracts and solvents such as vegetable oils, phenoxyethanolas non-alkanediol alcohol at 0.5-4%, and Triclosan for application tovarying surfaces.

US 2011/0300083 to Yontz et al. discloses a personal care formulation,that may be anhydrous, comprising antifungal agents and depilatorycompounds.

http://www.cosmetoscope.com/2011/07/olay-smooth-finish-facial-hair-removal-duo/disclosesfrom Olay a hair removal cream that is an oil/water emulsion withmineral oil and calcium thioglycolate hydroxide as depilatory.

http://www.silkn.com/Hair_Removal_Cream is a general discussion aboutdepilatories.

Other references include the following:

-   ASTM E2315-03(2008) Standard Guide for Assessment of Antimicrobial    Activity Using a Time-Kill Procedure. 100 Barr Harbor Drive, West    Conshohocken, Pa., USA. http://www.astm.org/Standards/E2315.htm-   National Committee for Clinical Laboratory Standards, Standard M7    A5: Methods for dilution antimicrobial susceptibility tests for    bacteria that grow aerobically. Fifth Edition, National Committee    for Clinical Laboratory Standards, Wayne, Pa., 1997.-   Winthrop K L, Abrams M, Yakrus M, et. al. An outbreak of    mycobacterial furunculosis associated with footbaths at a nail    salon. New England Journal of Medicine. 2002; 326(18):1366-1371.-   http://wwwnc.cdc.gov/eid/article/14/11/07-1297_article.htm-   Zafar A B, Butler R C, Reese D J, et. al. Use of 0.3% triclosan    (Bacti-Stat) to eradicate an outbreak of methicillin-resistant    Staphylococcus aureus in a neonatal nursery. American Journal of    Infection Control. 1995; 23(3): 200-208.

OBJECTS AND SUMMARY OF INVENTION

It is an object of this invention to provide an anhydrous, antibacterialhair removal composition and a procedure for manufacturing and usingsuch composition.

It is yet another object of this invention provide a non-aqueous, liquidhair removal composition that is effective and safe and can be used atrelatively low temperatures, e.g., less than 160° F. (71° C.) and havean effective shelf-life.

It is a further object of this invention to provide an anhydrous,antibacterial hair removal composition that does not stick to the skinbut to the oils of the hair and is erasable or can be completelydissolved with sunflower, safflower and/or mineral oil and will notharden unless it comes in contact with a hydrous composition, e.g.,water.

The foregoing objects are achieved with a non-aqueous antibacterial hairremoval composition of this invention that comprises an amount ofsolubilizing oil, an amount of botanical oils and rosins, e.g., soybeanoil, gum rosin, and rosin esters and an amount of an antibacterial agentselected from the group consisting of:

1. About 0.1% to about 0.5% by weight of the hair removal composition oftriclosan;

2. About 0.1% to about 2% by weight of the hair removal composition ofbenzethonium chloride; or

3. Mixtures thereof.

The amounts of botanical oils and rosins, such as soybean oil, gum rosinand rosin esters, in the non-aqueous hair removal composition aresufficient that when the hair removal composition is applied to aperson's skin and subsequently removed, hair on the skin is entrapped inthe hair removal composition and removed therewith. The amount ofsolubilizing oil is an effective amount for solubilizing the botanicaloils and rosins, e.g., soybean oil, gum rosin, rosin esters andantibacterial agent. The composition may also include additionalfragrances and additional bacteriocides, e.g., phenoxyethanol.

The hair removal composition is applied to a person's skin, and after asufficient amount time, removed from the person's skin with the hairentrapped therein.

The hair removal composition of this invention removes body hair gentlyand under lower temperatures than are required for wax and wax likeproducts, i.e., less than about 160° F. (71° C.), and has beenclinically tested to show a 99.97% reduction of bacteria. Thecompositions remain bacteria free at low temperatures of use.

Applicant describes herein numerous embodiments of the invention inconjunction with the drawings, which include variations in the differentelements of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS

The novel features believed to be characteristic of the presentinvention, together with further advantages thereof, will be betterunderstood from the following description considered in connection withthe accompanying drawings in which several embodiments of the inventionare illustrated by way of example. It is to be expressly understood,however, that the drawings are for the purpose of illustration anddescription only and are not intended as a definition of the limits ofthe invention.

FIG. 1 depicts contacting an eyebrow applicator with the hair removalcomposition of this invention.

FIG. 2 depicts applying the hair removal composition to a person'seyebrows with the applicator.

FIG. 3 depicts contacting a spatula type applicator with the hairremoval composition to provide an applicator having the hair removalcomposition thereon.

FIG. 4 depicts applying the hair removal composition to a person's legwith the applicator.

FIG. 5 depicts a technique for contacting an applicator with the hairremoval composition.

FIG. 6 depicts a detailed cross-sectional view applying the hair removalcomposition to a person's skin with the applicator.

DETAILED DESCRIPTION OF THE INVENTION

The foregoing objects are achieved with a non-aqueous antibacterial hairremoval composition of this invention that comprises an amount ofsolubilizing oil, an amount of botanical oils and rosins, such as anamount soybean oil, an amount of gum rosin, an amount of rosin estersand an amount of an antibacterial agent selected from the groupconsisting of:

1. About 0.1% to about 0.5% by weight of the hair removal composition oftriclosan;

2. About 0.1% to about 2% by weight of the hair removal composition ofbenzethonium chloride; and

3. Mixtures thereof.

The amounts of botanical oils and rosins, such as soybean oil, gum rosinand rosin esters, in the non-aqueous hair removal composition aresufficient that when the hair removal composition is applied to aperson's skin and subsequently removed, hair on the skin is entrapped inthe hair removal composition and removed therewith. The amount ofsolubilizing oil is an effective amount for solubilizing the botanicaloils and rosins and antibacterial agent.

As used herein when the term “weight percent” is used it means theweight of the referenced compound or composition used in the hairremoval composition.

Specifically preferred compositions include:

a. About 6.0% to about 8.0% by weight mineral oil, preferably about 6.5%by weight;

b. About 12.0% to about 16.0% by weight soybean oil, preferably about14% by weight;

c. About 6.0% to about 8.0% by weight gum rosin, preferably about 7.5%by weight;

d. About 68% to about 72% by weight rosin esters, preferably about 71%by weight;

e. About 0.1% to about 0.5% by weight triclosan, preferably about 0.3%by weight;

f. About 0.1% to about 2.0% by weight benzethonium chloride, preferablyabout 0.2% by weight;

g. About 0.1% to about 0.3% by weight titanium dioxide; and

h. About 0.1% to about 0.2% by weight fragrance.

The composition may also include additional fragrances andbacteriocides, e.g., phenoxyethanol.

The highly preferred hair removal composition of this invention utilizesFood Grade Rosin Ester, WW grade Gum Rosin, Soybean oil, White MineralOil, Benzethonium chloride (CAS #121-54-0),Triclosan(5-chloro-2-(2,4-dichlorophenox)phenol) and Titanium Dioxide.

The hair removal composition is applied to a person's skin, and after asufficient amount time, removed from the person's skin with the hairentrapped therein.

Unlike wax and non-wax related products in the marketplace used for hairremoval, the hair removal composition of this invention has beenclinically tested and shows a 99.97% reduction of bacteria. The hairremoval composition removes hair gently and under lower temperaturesthan are required for wax and wax like products that need constantheating above 72° C. (161° C.). This relatively high temperature cancause burning of the human skin tissue. Additionally, bacteria canquickly form in such products and be transferred to the skin if used alower temperatures.

The hair removal composition of this invention remains bacteria freewithout such high temperatures, stays liquid at body surfacetemperatures and does not cause lifting of skin tissue when removed.Because of its anhydrous nature, when applied to the body thecomposition couples and/or bonds to the sebaceous oil secreted but notto the person's skin.

The hair removal composition of this invention is completely dissolvedwith a safflower/sunflower erasing lotion causing no ill effects to theskin or clothing.

The hair removal composition is applied to the skin, e.g., see FIGS.1-6. The procedure is similar to waxing with several exceptions thatmake it safer and less painful. The product is removed with the hair,with a latex free, non-woven strip material. There is no breakage orstinging because the hair removal composition remains in a semi-liquidstate and does not dry on the skin.

The hair removal composition of this invention is not a chemicaldepilatory, which dissolves hair. The procedure is a form of manualdepilation with no breakage to the follicles and no risk of crosscontamination when used in the salon or professionals office on severalclients.

Referring to the Figures, the applicators used in this invention arepreferably medical grade stainless steel applicators. Although suchmaterial of construction for such applicators is preferred, thisinvention contemplates any type material, e.g., polymeric, plastic orwooden, that can be effectively and safely used to apply the hairremoval composition of this invention. In the Figures two types ofapplicators are depicted, i.e., a body applicator 10A, and an eyebrowapplicator 10B.

The preferred applicators 10 used in this invention are Japanese medicalgrade stainless steel that can be efficiently cleaned by thecompositions and methods described in U.S. Ser. No. 13/607,698 filed onSep. 8, 2012, now pending, entitled Compositions For CleaningApplicator's for Hair Removal Compositions.

The hair removal composition of this invention comprises solubilizingoil. The preferred oil for use in the hair removal composition of thisinvention is mineral oil, i.e., an insoluble cosmetic oil which alsoacts as emollient for the ingredients. It is a hydrophobic or waterrepellent ingredient. It is also non-toxic, colorless, transparent,odorless and tasteless, and, when heated, smells like petroleum.

Although mineral oils are preferred, other hydrocarbon oils may be usedsuch as rapeseed oil, theobroma oil, castor oil, jojoba oil, siliconeoil, lanolin, olive oil, cocoa butter, and shea butter and variousfragrances and essential oils selected to solubilize the specificingredients in the non-aqueous hair removal composition at relativelylow temperatures, i.e., commercially safe temperatures, and capable ofholding therein an effective amount of the selected antibacterial agentor agents.

Additional solubilizing oils that may be included in the hair removingcomposition of this invention are hydrocarbon-based emollients such aspetrolatum, fatty ester-based emollients, such as methyl, isopropyl andbutyl esters of fatty acids such as isopropyl palmitate, isopropylmyristate, isopropyl isostearate, isostearyl isostearate,diisopropylsebacate, and propylene dipelargonate, 2-ethylhexylisononoate, 2-ethylhexyl stearate, C(12)-C(16) fatty alcohol lactatessuch as cetyl lactate and lauryl lactate, isopropyl lanolate,2-ethylhexyl salicylate, cetylmyristate, oleylmyristate, oleyl stearate,oleyloleate, hexyl laurate, and isohexyllaurate.

In the preferred hair removing composition of this invention thesolubilizing oil is mineral oil that is present at from about 6.0% toabout 8.0% weight percent of the hair removing composition.

The hair removing composition further includes an effectiveantibacterial amount of an antibacterial agent. The preferred hairremoval composition of this invention includes one or more antimicrobialagents, preferably at a total concentration between 0.01% and 5% byweight, preferably between 0.05% and 3% by weight, and most preferablybetween about 0.1% and 2% by weight. In the preferred composition, thereis from about 0.1% to about 0.5%, preferably 0.3% by weight of triclosanand/or about 0.1% to about 0.5%, preferably about 0.2% by weight ofbenzethonium chloride, but as high as 2% by weight if triclosan is notused.

Triclosan is a chlorinated diphenyl ether. It is an antimicrobial agentthat has been employed for a variety of purposes for more than 20 years.Triclosan is preferred based on its efficacy and its solubility in theselected solubilizing oil. Triclosan has an exceedingly low solubilityin water, e.g., 5 to 10 ppm and a relatively high solubility in mineraloil.

Chemical Name: 2,4,4′Trichlorl-2′-Hydroxydiphenyl Ether

Phenol 5-chloro-2-(2,4-clichlorophenoxy) CAS Number 3380-34-5

Triclosan is relatively non-toxic to humans and other mammals. It isalso not found to have any carcinogenic, mutagenic or teratogeniceffects. It is used clinically, in oral hygiene products as well as in awide range of consumer products. Triclosan has a broad range of activitythat encompasses many, but not all types of Gram positive and Gramnegative non-sporulating bacteria, some fungi and viruses. Triclosan ismarketed by Ciba-Geigy of Basle, Switzerland, under the name ofIrgasan®.

The hair removal compositions of the invention may alternatively oradditionally include one or more antibacterial or anti-microbial agentsthat can be carried in, e.g., solubilized or emulsified, in the selectedsolubilizing oil. The agent should have low water content and/or notneed water so that it can blend with the selected solubilizing oil.

Examples of agents that may be used include, but are not limited to,chitosan, phenoxyethanol, chlorhexidinegluconate, iodophores, iodine,benzoic acid, dehydroacetic acid, propionic acid, sorbic acid, methylparaben, ethyl paraben, propyl paraben, butyl paraben, isobutyl paraben,cetrimide, chlorhexidine (free base and/or salts), other biguanides,chloroerosol, chloroxylenol, benzyl alcohol, bronopol, benzalkoniumchloride, benzethonium chloride, ethanol, phenoxyethanol, phenyl ethylalcohol, 2,4-dicholorobenzyl alcohol, thiomersal, clindamycin,erythromycin, benzoyl peroxide, mupirocin, bacitracin, polymixin B, DMDMHydantoin (Glydant), GERMALL, Kathon CG, Phenonip, miconazole,fluconazole, itraconazole, etcn-alkyl dimethyl benzyl ammonium chloride,n-alkyl dimethyl benzyl ammonium chloride, dialkyl dimethyl ammoniumchloride, didecyl dimethyl ammonium chloride, dioctyl dimethyl ammoniumchloride, phenolics, iodophors, pine oil, methyl salicylate, morpholine,silver, copper, bromine, and quaternary ammonium compounds, derivativesthereof, and combinations thereof. Antibacterial perfumes such as, forexample, thymol, thyme oil, eugenol, clove oil, menthol, mint oil,farnesol, and antibacterial glycerol esters such as, for example,glycerol monocaprate, glycerol monocaprylate, glycerol monolaurate (GML)and diglycerolmonocaprate (DMC) may also be used.

The preferred alternative or additional antibacterial agent isbenzethonium chloride. Benzethonium chloride is a synthetic quaternaryammonium salt. This compound is an odorless white solid; soluble inwater. It has surfactant, antiseptic, and anti-infective properties, andit is used as a topical antimicrobial agent in first aid antiseptics. Itis also found in cosmetics and toiletries such as mouthwashes, anti-itchointments, and antibacterial moist towelettes. Benzethonium chloride isalso used in the food industry as a hard surface disinfectant.

Benzethonium chloride exhibits a broad spectrum of microbiocidalactivity against bacteria, fungi, mold and viruses. Independent testingshows that benzethonium chloride is highly effective against suchpathogens as methicillin-resistant Staphylococcus aureus, Salmonella,Escherichia coli, Clostridium difficile, hepatitis B virus, hepatitis Cvirus, herpes simplex virus (HSV), human immunodeficiency virus (HIV),respiratory syncytial virus (RSV), and norovirus.

The US Food and Drug Administration (FDA) specifies that the safe andeffective concentrations for benzethonium chloride are 0.1-0.2% in firstaid products. Aqueous solutions of benzethonium chloride are notabsorbed through the skin. It is not approved in the US and Europe foruse as a food additive.

In addition to its highly effective antimicrobial activity, benzethoniumchloride contains a positively charged nitrogen atom covalently bondedto four carbon atoms. This positive charge attracts it to the skin andhair. This contributes to a soft, powdery after feel on the skin andhair, as well as long-lasting persistent activity againstmicroorganisms.

Benzethonium chloride is available under trade names Salanine, BZT,Diapp, Quatrachlor, Polymine D, Phemithyn, Antiseptol, Disilyn,Phermerol, and others.

The most common forms of bacteria and fungus which are the cause of mostirritations or infections are Staphylococcus aureus (ATCC 6538);Pseudomonas aeruginosa (ATCC 9027); and E. coli (ATCC 8739). Commonforms of fungi include Aspergillus niger (ATCC 16404); and Candidaalbicans (ATCC 10231).

Additional ingredients are added to the hair removal composition of thisinvention, for example fragrances. Fragrances that may be used inaccordance with the present invention include any synthetic as well asnatural fragrance and mixtures thereof. A multiplicity of fragrances maybe used to achieve the desired effect. Apart from their effects asfragrances, such compounds also may be useful in the instant inventionas antimicrobial agents and/or preservatives.

Typically, a fragrance comprises between about 0.1% by weight to about5.0% by weight of the final composition. Generally, the amount of suchfragrance is not critical.

Examples of synthetic fragrances that may be used in accordance with thepresent invention include without limitation acetanisole; acetophenone;acetyl cedrene; methyl nonyl acetaldehyde; musk anbrette; heliotropin;citronellol; sandella; methoxycitranellal; hydroxycitranellal; phenylethyl acetate; phenylethylisobutarate; gamma methyl ionone; geraniol;anethole; benzaldehyde; benzyl acetate; benzyl salicate; linalool;cinnamic alcohol; phenyl acetaldehyde; amyl cinnamic aldehyde; caphore;p-tertiairy butyl cyclohexyl acetate; citral; cinnamyl acetate; citraldiethyl acetal; coumarin; ethylene brasslate; eugenol; 1-menthol;vanillin; and mixtures thereof.

Examples of natural fragrances that may be used herein include, withoutlimitation, lavandin; heliotropin; sandlewood oil; oak moss; pathouly;ambergris tincture; ambrette seed absolute; angelic root oil; bergamontoil; benzoin; Siam resin; buchu leaf oil; cassia oil; cedarwood oil;cassia oil; castoreum; civet absolute; chamomile oil; geranium oil;lemon oil; lavender oil; YlangYlang oil; and mixtures thereof.

The preferred fragrance is about 0.1% to about 0.2% by weight SymocidePS ((Phenoxy Ethanol, 1,2 Hexanediol, Decylene Glycol).

The hair removal compositions of this invention may also contain apreservative or preservative system to inhibit the growth of pathogensover an extended period of time. The preferred preservative is Neolone100, which is phenoxyethanaol available from Dow. Other suitablepreservatives for use may include, Kathon CG, which is a mixture ofmethylchloroisothiazolinone and methylisothiazolinone available fromRohm & Haas; iodopropynylbutylcarbamate; benzoic esters (parabens), suchas methylparaben, propylparaben, butylparaben, ethylparaben,isopropylparaben, isobutylparaben, benzylparaben, sodium methylparaben,and sodium propylparaben; 2-bromo-2-nitropropane-1,3-diol; benzoic acid;imidazolidinyl urea; diazolidinyl urea; and the like. Still otherpreservatives may include ethylhexylglycerin (Sensiva SC 50 bySchulke&Mayr), phenoxyethanol (Phenoxyethanol by Tri-K Industries),caprylyl glycol (Lexgard O by Inolex Chemical Company, Symdiol 68T (ablend of 1,2-hexanediol, caprylyl glycol and tropolone by Symrise) andSymocide PT (a blend of phenoxyethanol and tropolone by Symrise).

The hair removal composition is applied to a person's skin, and after asufficient amount time, removed from the person's skin with the hairentrapped therein.

The process of manufacturing a preferred composition comprises:

a. Heating about one-half the amount of soybean oil to a temperature offrom about 85° C. (about 185° F.) to about 95° C. (about 203° F.) toproduce a heated soybean oil;

b. Mixing the amount of gum rosin in the heated soybean oil to producean initial emulsified composition;

c. Adding with constant stirring the remaining amount of soybean oil tothe emulsified composition to produce a final emulsified composition;

d. Cooling the final emulsified composition a temperature of about 80°C. (about 176° F.) to about 85° C. (about 185° F.) to produce a cooledfinal emulsified composition;

e. Mixing the amount of rosin esters into the cooled final emulsifiedcomposition for a period of at least one hour to produce a mixedcomposition;

f. Cooling the mixed composition to about 60° C. (about 140° F.) toabout 70° C. (about 158° F.) to produce a cooled mixed composition;

g. Mixing the amount of mineral oil, the amount of triclosan, and theamount of benzethonium chloride to form a second emulsion;

h. Mixing the second emulsion with the cooled mixed composition for aperiod of from 2 to 6 hours and subsequently cooling to room temperatureto produce the non-aqueous, antibacterial hair removal composition.

Referring to FIGS. 1-6, an applicator 10 is provided for applying thehair removal composition 18 to the skin, e.g., the leg L or eyebrow EB.Preferably, the applicator 10 is made of medical grade stainless steel.The use of a stainless steel applicator 10 also permits a finer coatingon the applicator 10 thus using less hair removal product 26. However,any of the well-known wood type applicators may be used.

Initially a sterile applicator 10 is contacted with the hair removalcomposition 26 to provide an applicator 10 having the hair removalcomposition on the tip 11A of the applicator 10. The applicator 10 isthen used for applying the hair removal composition 26 to a person'sskin.

Referring to FIGS. 1, 3 and 5, the applicator 10 is then dipped in ahair removal composition 26 held in heated container 24 and the excessis removed by passing the applicator 10 over the scraper bar 28 over theopening of container 24. In FIGS. 1, 3 and 5, a heater 24 is shown intowhich a hair removal product 26 is placed, e.g., a can containing theproduct, so that it can be heated. The excess hair removal product isscraped from the applicator 10 by a scraper bar 28, which can be fittedover an arc of the peripheral edge of the can or heater.

In FIGS. 1 and 2, the eyebrow applicator 10B tip 11A is used to applythe hair removal composition 26 to the eyebrow EB for subsequent removalfrom the eyebrow EB (not shown). In FIGS. 3-6, the body applicator 10A,which has a curved surface on both ends 11A, is used to apply the hairremoval composition 26, for example, to the leg L for subsequent removalfrom the eyebrow leg L (not shown) of the hair removal composition 26and the hair.

More specifically, referring to FIGS. 1 and 2, when the technician isready to use the eyebrow applicator 10B the applicator tip 11A is“tapped” into the hair removal composition 26 in heater unit 24 until alittle round ball of product is formed on the tip 11A. The tip is thencontacted with the scrapper bar 28 to produce a small “ball of product”of sufficient amount to do the whole eyebrow EB. The technician thenapplies a nice smooth path of product on the hair that needs to beremoved. The applicator 10 applies a thin, even coating of hair removalproduct 26 on the skin with no harm to the skin.

Again, more specifically, referring to FIGS. 3-6, when the technician isready to use the body applicator 10A he/she will dip the applicator intothe heated hair removal composition 26. The tip 11A of the applicator10A is then scrapped across the scraper bar 28 on one side of theapplicator tip 11A (FIG. 5) to clean that side of the applicator tip11A. The technician then applies the other side of the tip 11A havingthe hair removal product thereon to the skin, e.g., leg L, at between a45° angle and 90° to the skin L applying the product in a thin layeronto the skin L.

After application of the hair removal composition 26 to the body, theapplicator 10 is contaminated with a residual amount of the hair removalcomposition 26, primarily but not solely on the tip 11A. At this point,if the applicator is disposable, e.g., wood, the applicator isdiscarded. Alternatively, and preferably, the applicator is a stainlesssteel applicator and cleaned in accordance with the compositions andprocedures described in U.S. Ser. No. 13/607,698 filed on Sep. 8, 2012,now pending, entitled Compositions For Cleaning Applicator's for HairRemoval Compositions.

The heater cup 24 for the hair removal composition is designed to keepthe temperature of the hair removal composition between 125° F. (52° C.)and 140° F. (60° C.) and is heated by electricity from a 12 volt, 2.5amp source.

The hair removal composition of this invention is antibacterial andliquid at low temperatures. The compositions do not require the use ofpasteurizing temperatures necessary for wax or resin based hair removalcompositions typically used in “waxing” processes. The hair removalcompositions of this invention may be used below 160° F. (71° C.) andmaintain a relatively long shelf life.

The hair removal compositions of this invention never dry whenmaintained at body temperature. The composition is anhydrous and doesnot stick to the skin but binds or coalesces with the oils of the hair.This is unique compared to other waxes or wax like products. Thecomposition is erasable, i.e., it can be completely dissolved withsunflower, safflower or mineral oil and will not harden unless it comesin contact with a hydrous element.

This new formula goes beyond any waxlike products on the market in thatit not only has all the attributes above but it is an antibacterialanhydrous formula.

In summary, the hair removal composition of this invention provides acombination of benefits and advantages over the known hair removalcompositions, in particular:

-   -   100% botanical—no chemicals, no sugar, no wax.    -   Bonds with the hair only    -   Less painful to use than other formulations.    -   Removes the entire hair, root, bulb and shaft each time—no        stubble.    -   Liquifies at body temperature, easy super thin application that        gives more value per ounce. Stays Liquid. Never dries.    -   No prior preparation. Applies easily—no waiting. Put it on, take        it off.    -   It's odorless.    -   Can be used for 98% of the body.    -   The only Antibacterial/Antimicrobial & Anhydrous hair removal        system.    -   Is erasable. Easily removed from skin & hair with Erasing Lotion        if there is an error.    -   Clean hair removal station easily with Erasing Lotion.

EXAMPLES Antibacterial Activity

The following methods were used in the preparation and testing of theantibacterial activity of the Hair Removal Compositions of thisinvention:

Determination of Rapid Germicidal (Time Kill) Activity of AntibacterialProducts.

The activity of the antibacterial hair removal compositions of thisinvention were measured by the time kill method, whereby the survival ofchallenged organisms exposed to an antibacterial test composition weredetermined as a function of time. In these tests, a diluted aliquot ofthe hair removal composition was brought into contact with a knownpopulation of test bacteria for a specified time at a specifiedtemperature. The test hair removal composition was neutralized at theend of the time period, which arrests the antibacterial activity of thecomposition. The percent or, alternatively, log reduction from theoriginal bacteria population was calculated. This time kill method oftesting the activity of antibacterial products is known to those skilledin the art.

The compositions may be tested at any concentration from 0-100% byweight. The choice of which concentration to use was at the discretionof the investigator, and suitable concentrations are readily determinedby those skilled in the art. For example, viscous samples usually aretested at 50% by weight dilution, whereas non-viscous samples are notdiluted. The test sample was placed in a sterile 250 ml (15.26 cubicinches) beaker equipped with a magnetic stirring bar and the samplevolume was brought to 100 ml (6.102 cubic inches), if needed, withsterile deionized water. All testing was performed in triplicate, theresults were combined, and the average log reduction was reported.

The choice of contact time period was also at the discretion of theinvestigator. Any contact time period can be chosen. Typical contacttimes range from 15 seconds to 5 minutes, with 30 seconds and 1 minutebeing typical contact times. The contact temperature also can be anytemperature, typically room temperature, or about 25° C. (about 77° F.).

The bacterial suspension, or test inoculum, was prepared by growing abacterial culture on any appropriate solid media (e.g., agar). Thebacterial population was then washed from the agar with sterilephysiological saline and the population of the bacterial suspension wasadjusted to about 10(8) colony forming units per ml (cfu/ml).

The table below lists the test bacterial cultures used in the followingtests and includes the name of the bacteria, the ATCC (American TypeCulture Collection) identification number, and the abbreviation for thename of the organism used hereafter.

ASTM E2315, Standard Guide for Assessment of Antimicrobial ActivityUsing Time-Kill Procedure, is incorporated herein by reference. Thisprocedure was used to assess the in vitro reduction of a microbialpopulation of test organisms after exposure to a test material.

Example 1 Test Organism(s)

S. aureus ATCC 6538

Purpose:

Determination of the efficacy of hair removal composition of thisinvention as described herein (“NN-3”) that includes 0.3% by weighttriclosan and 0.5% by weight benzethonium chloride.

Experimental Design:

Test 1: Time Kill Study Using 1 Part Inoculum: 9 Parts Test Product, aModification of ASTM Method E2315.

The following test organisms were used:

1) S. aureus ATCC 6538

Note: inoculum is increased 10× compared to previous experiment todetermine if the percentage reduction is antibacterial (≧99.9%)

A 0.09 ml (0.005492 cubic inch) aliquot of NN-3 hair removal compositionwas warmed to 52° C. and mixed with 0.01 ml (0.0006102 cubic inch) of anovernight culture diluted to 0.45 OD at 600 nm, and mixed for 15seconds. After the time has elapsed, 4.9 ml (0.299 cubic inch) of D/Ebroth was added to the reaction tube to quench antimicrobial theactivity. A secondary 1:100 dilution with D/E broth (0.02 ml to 1.98 mlD/E broth) and 0.5 ml aliquots of the last dilution are plated. The TSAplates were incubated for 24-48 hours at 35° C. to 37° C. As a control,0.09 ml of NN-3 hair removal composition without triclosan andbenzethonium chloride was substituted for the test product and wasprocessed in the same manner as the test groups with an extra 1:10dilution of which, a 0.5 ml aliquot was plated. A saline+D/E brothun-inoculated blank was also subcultured to ensure sterility of themedia and technique.

Note: if applicable, the different test samples were tested alternatelyto eliminate an order bias, e.g. sample X was tested followed by sampleY and then sample Z and back to sample X, etc.

Calculations:

# of CFU/control sample: CFU/plate×10,000

# of CFU/test sample: CFU/plate×1000

Zone of Inhibition Assay Using S. Aureus ATCC 6538

An overnight culture of S. aureus was diluted to 0.46 OD at 600 nm andthen further diluted 10⁻² for inoculation of TSA plates as follows: 0.5ml was spread across the surface of a sterile TSA plate and allowed todry.

Materials:

-   -   1. Cole-Parmer D/E Neutralizing Broth Lot No: 10162 Expiration        Date: 5/2013    -   2. NN-3 including 0.3% triclosan+0.5% (w/w) benzethonium        chloride (Lonzagard BZT);    -   3. Difco TSA    -   4. Cole-Parmer TSB        Results:        Time Kill Study.        v. S. aureus ATCC 6538        Control 1 # of CFU/plate: 114 # of CFU/control sample: 1.1×10⁶        NN-3 Test 1 # of CFU/plate: 1 # of CFU/test 1 sample: 1.0×10³        NN-3 Test 2 # of CFU/plate: 4 # of CFU/test 2 sample: 2.0×10³        NN-3 Test 3 # of CFU/plate: 1 # of CFU/test 2 sample: 1.0×10³        Average counts/sample: 1.3×10³        Average reduction: 99.988%        Blank Sample # of CFU/plate: 0

Conclusions: The results of a repeat test of the combination of 0.3%triclosan+0.5% BZT match previous results, i.e., a reduction inchallenge organism counts of ≧99.9%.

Modification of ASTM E2315

TABLE 1 Compendium of NN-3 hair removal composition time kill results.Control NN-3 Test organism cfu/sample X cfu/sample % reduction S. aureus1.1 × 10⁶ 1.3 × 10³ 99.99% ATCC 6538

Example 2 Test Organism(s)

S. aureus ATCC 6538

Experimental Design:

Project Title: Determination of the Antimicrobial Activity of HairRemoval Composition Using Time Kill Method ASTM E2315-03.

Description of the Study

The risk of transmission of pathogens from one person to another is aconcern in the beauty salon industry where products, equipment and toolsof the trade may be re-used on multiple clients. Health departments andother regulatory bodies require that stringent hair removal andsanitization procedures are carried out on any non-disposable equipmentor materials such as scissors and re-useable product applicators inbetween clients. In some cases, equipment is “double dipped” inmaterials in between clients and it then becomes necessary to validatethat these materials are self-disinfecting. An example of such aantimicrobial material is “Barbicide®” that is used to disinfect combsand scissors in between clients.

This study was designed to determine if the preferred hair removalcomposition of this invention (NN-3) is self-disinfecting when used asdirected. In the salon setting, the hair removal composition is appliedto the skin from a heated reservoir using either a wood applicator thatis discarded after each client or a stainless steel applicator that iscleaned and disinfected in between clients.

In this study we investigated whether pathogens could survive insub-pasteurization temperatures (68° C. (154. ° F.) to 72° C. (161. °F.)) in the hair removal composition of this invention containingtriclosan.

General Description of the Assay

A modification of a time kill assay based on ASTM method E2315-03 andstandardized microbiological testing was used to determine the efficacyof a the hair removal composition of this invention containing triclosanas the antibacterial agent.

An aliquot of the composition was heated to 68° C. to 72° C. andinoculated with a known amount of challenge organism. After a 15 secondinterval the antimicrobial action was stopped using a validated druginactivating medium. As a control, a non-antimicrobial version of hairremoval composition, i.e., without triclosan was used at 45° C. (113°F.) in an otherwise identical test set-up. After appropriate dilutionsand plating, the surviving organisms were enumerated and the percentagereduction in the test group was determined by comparison to the control.

This model was designed to determine the capability of the hair removalcomposition of this invention in rapidly inactivating any organisms thatmay be introduced into the heated reservoir when the applicator wasdipped into it. If the hair removal composition of this inventiondemonstrates rapid and broad spectrum antimicrobial activity. Thisindicates that the risk of cross contamination among clients may bereduced or eliminated in the salon setting.

Materials and Methods

Materials:

1. Cole Parmer trypticase soy agar (TSA)

2. Cole-Parmer trypticase soy broth (TSB)

3. Sabouraud dextrose agar (SDA)

4. Sabouraud dextrose broth (SDB)

5. Cole-Parmer D/E Neutralizing Broth

6. Hair removal composition (triclosan-free; Lot # EQL-NN-0)

7. Hair removal composition (Lot # EQL-NN-1); custom prepared with 0.3%(weight/weight) triclosan.

8. Gamma sterilized culture tubes

9. Digital timer

10. Spectronic® 20

11. Autoclave

12. Vortex mixers

13. Gamma sterilized serological pipettes

14. Micropipettes and steam sterilized tips

15. Gamma sterilized Petri dishes

16. Lab magnetic stirrer/hotplates

17. ASTM Traceable® digital temperature probes

18. Constant temperature incubators

19. Test Organisms:

-   -   a. Staphylococcus aureus ATCC #6538    -   b. Pseudomonas aeruginosa ATCC #9027    -   c. Escherichia coli ATCC #8739    -   d. Methicillin Resistant Staphylococcus aureus ATCC #33592    -   e. Aspergillus brasiliensis ATCC #16404    -   f. Candida albicans ATCC #10231        Methods        Experimental Setup for ASTM Method E2315-03 In Vitro Time Kill        Assays:

A 0.09 ml aliquot of test hair removal composition of this invention waswarmed to 70° C.±2° C. was mixed with 0.01 ml of an overnight culture ofthe challenge organism (diluted to 0.45 OD at 600 nm with either TSB forbacteria or SDB for yeast and mold), and mixed thoroughly using the tipof the inoculating pipette for 15 seconds. After the time has elapsed,4.9 ml of D/E broth was added to the reaction tube to quench theantimicrobial activity. A secondary 1:100 dilution was carried out usingD/E broth 0.02 ml to 1.98 ml D/E broth—except for C. albicans—see rawdata) and 0.5 ml aliquots of this dilution were plated (TSA is used forbacteria and SDA for yeast and mold).

The plates were incubated for 24-48 hours at 35° C. to 37° C. forbacteria and yeast and for 5 days at 21° C. for fungus. Triplicatesamples were used in the test group. As a control, 0.09 ml of the hairremoval composition without triclosan was substituted for the testproduct and processed in the same manner as the test groups with thefollowing exceptions:

a) the control was kept at 45° C., and

b) an extra 1:10 dilution was carried out of which a 0.5 ml aliquot wasplated.

A medium+D/E broth uninoculated blank was also subcultured to ensuresterility of the media and technique.

Note: if applicable, the different test samples were tested alternatelyto eliminate an order bias, e.g. sample X was tested followed by sampleY and then sample Z and back to sample X, etc.

Validation of Test Parameters:

Demonstration of inactivation of residual triclosan in the first andsecond dilutions so that carry-over of drug does not produce a falsenegative. Briefly, D/E broth was inoculated with approximately 100CFU/ml of either Staphylococcus aureus ATCC #6538 or Pseudomonasaeruginosa ATCC #9027 and added to tubes containing 0.1 gram of testproduct. As a control, 0.1 gram of TSB was substituted for the testproduct. After vortexing the tubes at high speed, a 0.5 ml aliquot wasremoved from the tube and plated on the appropriate agar. The microbialcounts recovered from the test and control groups were compared. Ifthere is less than a 5% difference between test and control groups, theinactivation of the triclosan was considered to be successful andtherefore eliminates the possibility of a false negative due topotential carry over of the drug in the actual assay.

Zone of Inhibition Assay:

An overnight culture of MRSA ATCC 3592 is diluted to 0.46 OD at 600 nmand then further diluted 10⁻² to obtain approximately 3×10⁶ CFU/ml forthe inoculation of TSA plates as follows: a 0.5 ml volume was spreadacross the surface of a sterile TSA plate and allowed to dry. A 7 mmwell was made in the center of the agar plate and approximately 200microliters of either the test or control (no antibacterial agent) hairremoval composition was pipetted into the well. The plates wereincubated at 37° C. for 24 hours and the zones of inhibition weredetermined by measuring the diameter of the clear circular area aroundthe product filled well.

Calculations:# of CFU/control sample: CFU/plate×10,000# of CFU/test sample: CFU/plate×1000The number of colony forming units recovered from a test sample wascalculated as follows:# of CFU/sample=[CFU/plate]×[dilution factor]For example:

A test subculture plate inoculated with 0.5 ml of a 1:1000 dilution ofthe recovery medium contains 5 CFU therefore:# of CFU/sample=[5]×[1000]=5000or3.7 Log 10 CFU/sample

If no colonies appeared on the test agar plate subculture then themicrobial counts were assumed to be less than 1000 due to the dilutionfactor.

Results

Validation of Test Parameters:

There was less than a 5% difference in the number of organisms recoveredfrom inoculated D/E broth exposed to either the control or test samplesprior to plating. If there was any carry-over of triclosan the D/E brothappears to have successfully quenched its antimicrobial properties.

ASTM Method E2315-03 In Vitro Time Kill Assays:

TABLE 1 Results expressed as % reduction of microbes in the test groupversus the control group after a 15 second exposure. Control Sample TestSample: EQL-NN-1 Challenge Organism cfu/sample cfu/sample % reductionStaphylococcus aureus 7.12 × 10⁶  <10³ >99.99 ATCC # 6538 Pseudomonas9.8 × 10⁶ <10³ >99.99 aeruginosa ATCC # 9027 Escherichia coli 2.7 × 10⁶<10³ >99.96 ATCC # 8739 MRSA 5.4 × 10⁶ <10³ >99.98 ATCC # 33592Aspergillus brasiliensis 3.4 × 10⁵ <10² >99.97 ATCC 16404 Candidaalbicans ATCC 4.7 × 10⁵ <10  >99.99 10231

TABLE 2 Results expressed as Log 10 reduction in microbial counts in thetest groups compared to those in the control group after a 15 secondexposure. Control Test Sample: Sample EQL-NN-1 Log 10 Log 10 Log 10Challenge Organism cfu/sample cfu/sample reduction Staphylococcus aureus6.85 <3.0 >3.85 ATCC # 6538 Pseudomonas aeruginosa 6.99 <3.0 >3.99 ATCC# 9027 Escherichia coli 6.43 <3.0 >3.43 ATCC # 8739 MRSA 6.73 <3.0 >3.73ATCC # 33592 Aspergillus brasiliensis 5.53 <2.0 >3.53 ATCC 16404 Candidaalbicans 5.67 <1.0 >4.67 ATCC # 10231

The results indicate that the hair removal composition of this invention(EQL-NN-1) formulation tested in this study meets the criteria forantibacterial and antifungal claims. EQL-NN-1 exhibited a greater than99.9% or 3 Log 10 reduction of a broad spectrum of organism in 15seconds.

TABLE 3 Zone of inhibition study using MRSA ATCC # 33592 as the testorganism. Test Group Zone of Inhibition (mm) EQL-NN-1 21 Control(EQL-NN-0) 0

FIG. 1. Zone of inhibition of EQL-NN-1 against MRSA ATCC #33592.

DISCUSSION AND CONCLUSIONS

One of the major concerns of product manufacturers and end users ofbeauty products such as salons is the risk of microbial contaminationand indeed, outbreaks of infections have been documented. In order tominimize this risk, the industry uses various strategies includingpreservatives, single use products and cleaning/sanitization procedures.

In the case of the hair removal composition of this invention, theproduct is kept free of microbial contamination by a combination ofchemical preservation and elevated temperatures. This study is designedto validate the efficacy of using a combination of temperature andchemical disinfection in reducing or eliminating a broad spectrum ofpathogenic organisms.

The hair removal composition of this invention that was tested contained0.3% triclosan uniformly dispersed throughout the product. Triclosan isa phenolic-based antimicrobial that is commonly found in a variety ofconsumer products such as soaps, deodorants and toothpaste. Althoughtriclosan is slow acting, it appears that when tested at highertemperatures such as in a range of 68° C. to 72° C. as was done in thisstudy, the antimicrobial activity of the product is greatly enhanced sothat a significant reduction in pathogens levels is achieved within 15seconds.

In conclusion, it appears that when the hair removal composition of thisinvention was used as directed in a salon setting, any potentialpathogenic organism introduced into the product will be rapidlyinactivated in 15 seconds or less. The results of this in vitro studyvalidate that hair removal composition of this invention formulated withtriclosan was rapidly self disinfecting.

In addition, the antimicrobial hair removal composition of thisinvention shows good activity against Methicillin resistantStaphylococcus aureus (MRSA) as demonstrated by a large zone ofinhibition. MRSA is prevalent in healthcare facilities and appears to bespreading into the general community. Triclosan has been shown to be anefficacious agent for controlling outbreaks of these pathogens.

As this and previous studies have shown, use of the hair removalcomposition of this invention antimicrobial has a very low probabilityof transmission of pathogens via cross contamination in the salonsetting.

What is claimed:
 1. A non-aqueous antibacterial hair removal compositioncomprising botanical oils and rosins, an amount of solubilizing oil andan antibacterial agent benzethonium chloride in an amount about 0.1% toabout 2.0% by weight of the hair removal composition, wherein the amountof botanical oils and rosins in the non-aqueous hair removal compositionare sufficient that when the hair removal composition is applied to aperson's skin and subsequently removed, hair on the skin is entrapped inthe hair removal composition and removed therewith; and wherein theamount of solubilizing oil is effective for solubilizing the amounts ofbotanical oils and rosins and antibacterial agent.
 2. A non-aqueousantibacterial hair removal composition comprising: a. An amount ofsolubilizing oil; b. An amount of soybean oil; c. An amount of gumrosin; d. An amount of rosin esters; e. antibacterial agent benzethoniumchloride in an amount of about 0.1% to about 2% by weight of the hairremoval composition, wherein the amount of soybean oil, gum rosin androsin esters in the non-aqueous hair removal composition are sufficientthat when the hair removal composition is applied to a person's skin andsubsequently removed, hair on the skin is entrapped in the hair removalcomposition and removed therewith; and wherein the amount ofsolubilizing oil is effective for solubilizing the amounts of soybeanoil, gum rosin, rosin esters and antibacterial agent.
 3. The hairremoval composition of claim 1, wherein the solubilizing oil is mineraloil.
 4. The hair removal composition of claim 1, further comprising acolorant.
 5. The hair removal composition of claim 1, further comprisingan effective amount of fragrance.
 6. The hair removal composition ofclaim 1, further comprising an amount of titanium dioxide.
 7. The hairremoval composition of claim 1, wherein the hair removal composition isliquid at temperatures above 25° C. (77° F.).
 8. A non-aqueousantibacterial hair removal composition comprising: a. An amount ofsolubilizing oil of about 6.0% to about 8.0%, wherein the solubilizingoil is mineral oil; b. An amount of soybean oil of about 12.0% to about16.0%; c. An amount of gum rosin of about 6.0% to about 8.0%; d. Anamount of rosin esters of about 68% to about 72%; e. An amount oftitanium dioxide of about 0.1%% to about 0.3%; f. An antibacterial agentbenzethonium chloride in an amount of about 0.1% to about 2.0% by weightof the hair removal composition wherein the amounts of soybean oil, gumrosin and rosin esters in the non-aqueous hair removal composition aresufficient that when the hair removal composition is applied to aperson's skin and subsequently removed, hair on the skin is entrapped inthe hair removal composition and removed therewith; and wherein thesolubilizing oil is mineral oil and the amount of solubilizing oil iseffective for solubilizing the amounts of soybean oil, gum rosin, rosinesters and antibacterial agent.
 9. A process of manufacturing thenon-aqueous antibacterial hair removal composition of claim 1,comprising: a. Heating about one-half the amount of soybean oil to atemperature of from about 85° C. (about 185° F.) to about 95° C. (about203° F.) to produce a heated soybean oil; b. Mixing the amount of gumrosin in the heated soybean oil to produce an initial emulsifiedcomposition; c. Adding with constant stirring the remaining amount ofsoybean oil to the emulsified composition to produce a final emulsifiedcomposition; d. Cooling the final emulsified composition a temperatureof about 80° C. (about 176° F.) to about 85° C. (about 185° F.) toproduce a cooled final emulsified composition; e. Mixing the amount ofrosin esters into the cooled final emulsified composition for a periodof at least one hour to produce a mixed composition; f. Cooling themixed composition to about 60° C. (about 140° F.) to about 70° C. (about158° F.) to produce a cooled mixed composition; g. Mixing the amount ofmineral oil and the amount of benzethonium chloride; h. Mixing thesecond emulsion with the cooled mixed composition for a period of from 2to 6 hours and subsequently cooling to room temperature to produce thenon-aqueous antibacterial hair removal composition.
 10. The process ofclaim 9, wherein the second emulsion further includes an amount oftitanium dioxide.
 11. A process of manufacturing the non-aqueousantibacterial hair removal composition of claim 9, comprising: a.Heating about one-half the amount of soybean oil to a temperature offrom about 85° C. (about 185° F.) to about 95° C. (about 203° F.) toproduce a heated soybean oil; b. Mixing the amount of gum rosin in theheated soybean oil to produce an initial emulsified composition; c.Adding with constant stirring the remaining amount of soybean oil to theemulsified composition to produce a final emulsified composition; d.Cooling the final emulsified composition a temperature of about 80° C.(about 176° F.) to about 85° C. (about 185° F.) to produce a cooledfinal emulsified composition; e. Mixing the amount of rosin esters intothe cooled final emulsified composition for a period of at least onehour to produce a mixed composition; f. Cooling the mixed composition toabout 60° C. (about 140° F.) to about 70° C. (about 158° F.) to producea cooled mixed composition; g. Mixing the amount of mineral oil and theamount of benzethonium chloride and titanium dioxide to produce a secondemulsion; h. Mixing the second emulsion with the cooled mixedcomposition for a period of from 2 to 6 hours and subsequently coolingto room temperature to produce the non-aqueous antibacterial hairremoval composition.
 12. The non-aqueous antibacterial hair removalcomposition of claim 1, further including 0.05% to about 3.0% of thymeoil.
 13. The non-aqueous antibacterial hair removal composition of claim1, further including an effective amount of a preservative ofphenoxyethanol.